The utility of the deuterated spin label in the spin immunoassay of cortisol.

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Abstract
A deuterated spin-labeled derivative of cortisol (F3-SLD) was prepared by coupling cortisol-3-(O-carboxymethyl)oxime with 4-amino-1-oxyl-2,2,6,6-tetramethylpiperidine-d17 and used to improve the sensitivity of spin immunoassay (SIA) of cortisol [in humans]. The linewidth of the high-field peak of F3-SLD was 1.4 G at the modulation amplitude (Hm) of 1.6 G, and that of the undeuterated spin-label was 1.9 G at Hm = 2.0 G. When 200 .mu.l of cortisol standards was used for the assay, the minimum detectable concentration of cortisol was reduced from 10 ng/200 .mu.l to 2.5 ng/200 .mu.l by the use of F3-SLD. Although the increase in the sensitivity of SIA is not yet sufficient, the use of deuterated spin-labels is considered to be useful for enhancing SIA sensitivity.

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