Regulation of enzyme formation in Klebsiella aerogenes by episomal glutamine synthetase of Escherichia coli

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Abstract
The physiology of cells of K. aerogenes containing the structural gene for glutamine synthetase (glnA) of E. coli on an episome was studied. The E. coli glutamine synthetase functioned in cells of K. aerogenes in a manner similar to that of the K. aerogenes enzyme; it allowed the level of histidase to increase and that of glutamate dehydrogenase to decrease during N-limited growth. The phenotype of mutations in the glnA site was restored to normal by the introduction of the episomal glnA+ gene. These results are consistent with the hypothesis that glutamine synthetase regulates the function of its own structural gene.