Regulation of Escherichia coli secA mRNA translation by a secretion-responsive element
Open Access
- 1 October 1991
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 173 (20) , 6605-6611
- https://doi.org/10.1128/jb.173.20.6605-6611.1991
Abstract
The Escherichia coli secA gene, whose translation is responsive to the proficiency of protein export within the cell, is the second gene in a three-gene operon and is flanked by gene X and mutT. By using gene fusion and oligonucleotide-directed mutagenesis techniques, we have localized this translationally regulated site to a region at the end of gene X and the beginning of secA. This region has been shown to bind SecA protein in vitro. These studies open the way for a direct investigation of the mechanism of secA regulation and its coupling to the protein secretion capability of the cell.Keywords
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