Alternative use of 5' exons in the specification of Ly-5 isoforms distinguishing hematopoietic cell lineages.
Open Access
- 1 August 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (15) , 5364-5368
- https://doi.org/10.1073/pnas.84.15.5364
Abstract
Previous inferences that Ly-5 glycoprotein isoforms of murine hematopoietic cells are generated by alternative splicing of primary transcripts of a single Ly-5 gene are supported by the present study. A cDNA library was prepared from B cells by extension from primer representing a known T-cell cDNA sequence. Three different Ly-5 clones from this library included sequences missing in T-cell cDNA clones. From the constitution of cDNA clones and of the Ly-5 gene, and from S1 nuclease mapping, it is concluded that at least two exons, provisionally numbered Ex-6(B) and Ex-7(B), in the 5''-proximal region are mainly represented in mRNA of the B-cell lines examined but not of the T-cell lines examined. Also, exons 1 and 2 appear to be used alternatively in different species of B-cell mRNA and probably also in different species of T-cell mRNA.This publication has 18 references indexed in Scilit:
- B-cell variant of mouse T200 (Ly-5): evidence for alternative mRNA splicing.Proceedings of the National Academy of Sciences, 1987
- Sequences of Ly-5 cDNA: isoform-related diversity of Ly-5 mRNA.Proceedings of the National Academy of Sciences, 1986
- Cloning of Ly-5 cDNA.Proceedings of the National Academy of Sciences, 1985
- Cell-type-specific cDNA probes and the murine I region: the localization and orientation of Ad alpha.Proceedings of the National Academy of Sciences, 1984
- A simple and very efficient method for generating cDNA librariesGene, 1983
- Different forms of Ly-5 within the T-cell lineageImmunogenetics, 1983
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Differences in the surface proteins of mouse B and T cells.Proceedings of the National Academy of Sciences, 1975
- Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-CelluloseProceedings of the National Academy of Sciences, 1972