Megakaryocyte differentiation capacity of human pluripotent bone marrow progenitor cells CFU‐GEMM in vitro after cryopreservation*

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Abstract
The effect of cryopreservation on the pluripotent haemopoietic progenitors CFU‐GEMM as well as on the megakaryocyte (CFU‐Mk), erythroid (BFU‐E) and granulocytic‐monocytic (CFU‐GM) progenitor cells was analyzed. Progenitor cell recovery after freezing, as determined in 5 experiments, averaged 89% for CFU‐GEMM (range: 63% ‐ 194%), 85% for CFU‐Mk (range: 62% ‐ 96%), 92% for BFU‐E (range: 43% ‐ 174%) and 60% for CFU‐GM (range: 31% ‐ 93%). Immunological analysis of individual mixed colonies using a double labelling immunoalkaline phosphatase slide technique and monoclonal antibodies against megakaryocytic and granulocytic cells revealed megakaryocyte cells in more than 79% (range: 73% ‐ 94%) and 84% (range: 75% ‐ 87%) of mixed colonies before and after freezing, respectively. Our results indicate that cryopreservation of human bone marrow cells does not alter the megakaryocytic differentiation capacity of the haemopoietic progenitor cells CFU‐GEMM and CFU‐Mk in vitro.