Comparison of Bioassay, High-Performance Liquid Chromatography, and Fluorescence Polarization Immunoassay for Quantitative Determination of Vancomycin in Serum

Publisher Website
Google Scholar
Abstract
Vancomycin, a glycopeptide antibiotic first isolated from Streptomyces orientalis, is in widespread clinical use for the treatment of penicillinase-producing staphylococcal infections. This investigation was designed to compare 3 assay techniques, the traditional bioassay (agar diffusion), and 2 more recent techniques, high-performance liquid chromatography (HPLC) and fluorescence polarization immunoassay (FPIA), for the determination of vancomycin concentrations in serum. One hundred clinical samples obtained from patients receiving vancomycin were assayed by each method. The results from each assay were compared using linear regression analysis. The resultant correlation coefficients were as follows: 0.9996 for the HPLC vs. FPIA, 0.773 for the FPIA vs. bioassay, and 0.7779 for HPLC vs. bioassay. The FPIA technique was the easiest and fastest of the 3 methods; FPIA and HPLC were the most accurate.

This publication has 2 references indexed in Scilit: