Practical Method for Estimating Oxygen Kinetic and Metabolic Parameters
- 1 March 1990
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 6 (2) , 164-167
- https://doi.org/10.1021/bp00002a012
Abstract
A practical and simple method was developed to determine the oxygen kinetic and metabolic parameters of cell cultures. The said parameters are the oxygen kinetic constant (Ko2), the maximum specific oxygen uptake rate (qmax), the maximum oxygen yield (Y), the critical dissolved oxygen (DO) (Ccr), and the oxygen maintenance coefficient (mo2). These parameters can be calculated from the results of one batch fermentation run. The fermentation needs to be run at an oxygen‐limiting condition, and the profiles of DO and cell density need to be recorded. A demonstration of the use of this method was done by using a yeast, Kluyveromyces marxianus.This publication has 6 references indexed in Scilit:
- Design of a system for the control of low dissolved oxygen concentrations: Critical oxygen concentrations for Azotobacter vinelandii and Escherichia coliBiotechnology & Bioengineering, 1985
- Influence of dissolved oxygen concentration on the oxygen kinetics of Gluconobacter oxydansApplied Microbiology and Biotechnology, 1985
- Influence of oxygen on the growth of Saccharomyces cerevisiae in continuous cultureBiotechnology & Bioengineering, 1983
- Calculation of the oxygen required for cell productionBiotechnology & Bioengineering, 1971
- Dynamic measurement of the volumetric oxygen transfer coefficient in fermentation systemsBiotechnology & Bioengineering, 1967
- The Measurement of the Aeration of Culture MediaJournal of General Microbiology, 1951