A Safe Method of Handling Mosquitoes for Virus Transmission Experiments

Publisher Website
Google Scholar
Abstract
Female pupae of Culex quinquefasciatus are placed in a cylindrical card board carton, both ends of which have been replaced with bobbinet. One end of the carton is then set deeply enough into water to allow the pupae to swim freely. After emergence, the carton is taken out of the water and a count of live adults made by subtracting the number of dead pupae from the total introduced. The specimens are fed on host animals through the bobbinet. Those failing to feed are removed by aspirator through a side-hole in the carton and counted so that the number of engorged specimens can be determined. Mosquitoes have been kept alive in these cartons for more than 60 days when properly humidified and provided periodically with soaked raisins.

This publication has 0 references indexed in Scilit: