Influence of the Nutrient Medium on the Recovery of Dividing Cells from Tobacco Protoplasts
- 1 March 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 57 (3) , 424-429
- https://doi.org/10.1104/pp.57.3.424
Abstract
Systematic tests resulted in a nutrient solution containing the following, in milligrams per liter, for the culture of protoplasts isolated from Nicotiana tabacum L. callus cells: Murashige and Skoog salts (T. Murashige and F. Skoog, 1962); sucrose, 15,000; mannitol, 110,000; .alpha.-naphthaleneacetic acid, 0.6; kinetin, 0-0.1; thiamine.cntdot.HCl, 10; pyridoxine.cntdot.HCl, 10; nicotinic acid, 5; myo-inositol, 100; and glycine, 2. In this medium, cell wall regeneration was observed in 85%, and resumption of cell division among 35% of the protoplast isolates.This publication has 6 references indexed in Scilit:
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