A C14 Collection System for Cattle and Its Effect on EKG and Respiratory Patterns

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Abstract
A metabolism unit for carbon-14 studies with the features of those described by Bergman (1964) and Robbins and Bakke (1967) was adapted to beef cattle. The system for collecting respiratory C14O2 was quantitative. Ninety percent of the C14O2 introduced into the system was recovered and the excretion patterns of C14O2 from animals injected with labeled glucose corresponded to other data reported in the literature. Electrocardiogram and pneumogram patterns and body temperature were recorded as indicators of physiological stress during the collection of expired CO2. At a room temperature varying from 23 to 29° C, body temperature and respiration rate increased during a 5-hr, collection period. Electrocardiogram patterns were unchanged. Under conditions of no apparent stress (room temperatures of 10.5 to 23° C.) respiration rate varied from one recording to the next and probably accounts for part of the error variation in the C14O2 activity of the respiration samples.

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