Exposure level monitor of 3‐amino‐1,4‐dimethyl‐5H‐pyrido[4, 3‐b]indole, a dietary carcinogen, in rabbits
- 1 January 1989
- journal article
- research article
- Published by Wiley in Environmental and Molecular Mutagenesis
- Vol. 14 (1) , 34-41
- https://doi.org/10.1002/em.2850140107
Abstract
The present investigation describes a method for the detection of minute amounts of 3‐amino‐1, 4‐dimethyl‐5H‐pyrido[4, 3‐b]indole (Trp‐P‐1), a carcinogenic tryptophan pyrolysate, bound to the hemoglobin of erythrocytes and plasma from rabbits dosed orally with the dietary carcinogen. The method consists of the acid‐induced release of the dietary carcinogen adducts as the free carcinogen and their extraction with methylene chloride and subsequent quantitation by high‐performance liquid chromatography (HPLC). With this method, Trp‐P‐1 levels in plasma, platelets, and red blood cells were monitored for 12 weeks to determine a suitable indicator for estimating the exposure levels of the dietary carcinogen. The results suggest that Trp‐P‐1 in red blood cells that bound covalently to the hemoglobin is the most suitable for monitoring the long‐term exposure levels.Keywords
This publication has 30 references indexed in Scilit:
- Carcinogenic tryptophan pyrolysis products in airborne particles and rain waterMutation Research Letters, 1989
- In vitro and in vivo N-acetylation of carcinogenic glutamic acid pyrolysis products in humansCarcinogenesis: Integrative Cancer Research, 1988
- Detection of Trp-P-1 and Trp-P-2, carcinogenic tryptophan pyrolysis products, in dialysis fluid of patients with uremiaMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1987
- Estimation of exposure of man to substances reacting covalently with macromoleculesArchives of Toxicology, 1987
- Presence of 2-amino-3,8-dimethylimidazo [4,5-f] quinoxaline (MeIQx) in dialysate from patients with uremiaBiochemical and Biophysical Research Communications, 1986
- Studies on Environmental Chemical Carcinogenesis in JapanScience, 1986
- Binding of orally given 2-amino-6-methyldipyrido (1,2-a:3',2'-d) imidazole, a carcinogen, to rat hemoglobin.Proceedings of the Japan Academy, Series B, 1986
- Biological aspects to the evaluation of risk: Dosimetry of carcinogens in manFundamental and Applied Toxicology, 1984
- Dosimetry of Electrophilic Compounds by Means of Hemoglobin AlkylationAnnual Review of Public Health, 1983
- Behaviour of Mammalian Hæmoglobin after Freezing and ThawingNature, 1963