A novel bend of DNA CIT: changeable bending-center sites of an intrinsic curvature under temperature conditions

Abstract
We found a novel DNA curvature, which has changeable bending-center sites of an intrinsic curvature under temperature conditions (CIT) in the cyanobacterium strain Microcystis aeruginosa K-81. Circular permutation analyses (CPA) for CIT under different temperature conditions (4–50°C) revealed that the changeable bending-center sites are located in the 5′-upstream region (−141 to −184) of the psbA2 gene, encoding the D1 protein homolog for photosynthesis. The nucleotide sequence around the bending center contains several dT (deoxy thymine) tracts, which seem to be a pivotal determinant for CIT.

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