Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain
- 7 March 1995
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 361 (2-3) , 238-242
- https://doi.org/10.1016/0014-5793(95)00189-g
Abstract
The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferritins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melitensis Hin dIII-digested genomic DNA identified a 3.8 kb fragment. This fragment was shown to contain a bacterioferritin gene ( bfr ) encoding a 161-amino acid protein. The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli , and many of the ferroxidase centre and haem-ligation residues are conserved.Keywords
This publication has 30 references indexed in Scilit:
- Complete sequence of the gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticumGene, 1994
- Structure of a unique twofold symmetric haem-binding siteNature Structural & Molecular Biology, 1994
- Purification, characterization, gene sequence, and significance of a bacterioferritin from Mycobacterium leprae.The Journal of Experimental Medicine, 1994
- Macrophage control of Brucella abortus: influence of cytokines and ironTrends in Microbiology, 1993
- Structure, function, and evolution of ferritinsJournal of Inorganic Biochemistry, 1992
- Physical, chemical and immunological properties of the bacterioferritins of Escherichia coli, Pseudomonas aeruginosa and Azotobacter vinelandiiBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1991
- Simultaneous and multivariate alignment of protein sequences: correspondence between physicochemical profiles and structurally conserved regions (SCR)Protein Engineering, Design and Selection, 1991
- Nitrobacter winogradskyi cytochrome b-559: a nonhaem iron-containing cytochrome related to bacterioferritinBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1989
- Amino acid sequence of the bacterioferritin (gytochrome b1) of Escherichia coli-K12Biochemical and Biophysical Research Communications, 1989
- The immune response of goats vaccinated with low and high doses of Rev 1Veterinary Immunology and Immunopathology, 1982