Expression of cell-cycle-dependent genes in phytohemagglutinin-stimulated human lymphocytes.
- 1 August 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (16) , 5375-5379
- https://doi.org/10.1073/pnas.82.16.5375
Abstract
We have investigated the expression of certain cell-cycle-dependent genes in human peripheral blood mononuclear cells (PBMC) stimulated by phytohemagglutinin (PHA). The genes studied had been previously identified as cell-cycle dependent in other cell types from different species and were induced by different mitogens. One of these genes (2F1) and the gene for the interleukin 2 receptor were induced by PHA even in cultures partially depleted of accessory cells where the lymphocytes grew in size but failed to enter S phase. The other genes (c-myc, 4F1, JE-3, and KC-1) were induced only in complete cultures of PBMC stimulated by PHA. These results confirm the dissociation between growth in size and cell DNA replication that can occur during cell-cycle progression. Moreover, the time course of appearance of detectable levels of RNA for these genes suggests that they may be used as markers of cell-cycle progression in the transition of lymphocytes from G0 to S phase.This publication has 50 references indexed in Scilit:
- Induction of c-fos gene and protein by growth factors precedes activation of c-mycNature, 1984
- Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and proteinNature, 1984
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984
- Simian Sarcoma Virus onc Gene, v- sis , Is Derived from the Gene (or Genes) Encoding a Platelet-Derived Growth FactorScience, 1983
- Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virusNature, 1983
- T-lymphocyte/monocyte interaction in response to phytohemagglutininCellular Immunology, 1979
- Correlation between cell cycle duration and RNA contentJournal of Cellular Physiology, 1979
- The ovalbumin gene: Alleles created by mutations in the intervening sequences of the natural geneCell, 1979
- Immunofluorescent studies of the development of pre‐B cells, B lymphocytes and immunoglobulin isotype diversity in humansEuropean Journal of Immunology, 1977
- Temperature sensitive mutants of BHK cells affected in cell cycle progressionJournal of Cellular Physiology, 1977