SPECIFIC LYSIS OF ANTIGENICALLY IRRELEVANT CELLS BY CYTOTOXIC T LYMPHOCYTES UPON INSERTION OF APPROPRIATE ANTIGENS INTO THE TARGET CELL PLASMA MEMBRANES

Publisher Website
Google Scholar
Abstract
The importance of the membrane milieu to functional presentation of target cell (TC) antigens to cytotoxic T lymphocytes (CTL) was investigated by examining the interaction of CTL with [mouse] TC plasma membrane (PM) fractions, in isolated form or integrated into antigenically irrelevant TC. Isolated ascitic vesicles, microsomes and purified PM, containing serologically defined alloantigens that have been implicated as the relevant TC antigens, effectively, yet nonspecifically, inhibited the binding and lysis of TC by CTL. The same PM fractions, when inserted into antigenically irrevelant TC via vesicles containing Sendai virus components, rendered the TC susceptible to CTL-mediated lysis directed against the inserted antigens. Evidently, CTL interact specifically with TC determinants only when they were embedded in the proper membrane environment.

This publication has 1 reference indexed in Scilit: