Inhibition of terminal deoxynucleotidyl transferase by adenine dinucleotides
- 9 March 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 213 (1) , 204-208
- https://doi.org/10.1016/0014-5793(87)81492-8
Abstract
Terminal deoxynucleotidyltransferase (TdT) exhibits strong sensitivity to ATP and its dinucleotide analogues, Ap2A, Ap3A, Ap4A, Ap5A and Ap6A. Similar to ATP, all of the dinucleotides appear to be competitive inhibitors of TdT catalysis with respect to substrate deoxynucleoside triphosphates and effectively block the UV‐mediated substrate cross‐linking to TdT. Among the various dinucleotides, Ap5A and Ap6A (diadenosine 5'‐5' penta‐ and hexaphosphate, respectively) are significantly more effective than dinucleotides containing 2, 3 or 4 phosphate backbones. Furthermore, Ap5A is found to be the only dinucleotide which has reactivity at both substrate‐ and primer‐binding domains in TdT.Keywords
This publication has 18 references indexed in Scilit:
- Fluorimetric assay for terminal deoxynucleotidyl transferase activityAnalytical Biochemistry, 1985
- Biochemistry of terminal deoxynucleotidyltransferase: characterization and properties of photoaffinity labeling with 8-azidoadenosine 5'-triphosphateBiochemistry, 1983
- The mechanism of inhibition of avian myeloblastosis virus reverse transcriptase by a dialdehyde derivative of ATP Inactivation of essential sulfhydryl group functionBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1983
- Synthesis of compositionally unique DNA by terminal deoxynucleotidyl transferaseBiochemical and Biophysical Research Communications, 1983
- Biochemistry of terminal deoxynucleotidyltransferase: identification, characterization, requirements, and active-site involvement in the catalysis of associated pyrophosphate exchange and pyrophosphorolytic activityBiochemistry, 1980
- Biochemistry of terminal deoxynucleotidyltransferase. Mechanism of manganese-dependent inhibition by deoxyadenosine 5'-triphosphate and biological implicationsBiochemistry, 1979
- Biochemistry of terminal deoxynucleotidyltransferase: mechanism of inhibition by adenosine 5'-triphosphateBiochemistry, 1978
- Kinetics of Inhibition of Deoxynucleotide‐Polymerizing Enzyme Activities from Normal and Leukemic Human Cells by 9‐β‐D‐Arabinofuranosyladenine 5′‐Triphosphate and 1‐β‐D‐Arabinofuranosylcytosine 5′‐TriphosphateEuropean Journal of Biochemistry, 1977
- Selective inhibition of terminal deoxynucleotidyl transferase (TdT) by adenosine ribonucleoside triphosphate (ATP) and its application in the detection of TdT in human leukemiaBiochemical and Biophysical Research Communications, 1977
- Observations on the pyridoxal 5'-phosphate inhibition of DNA polymerasesBiochemistry, 1976