Genes for the eight ribosomal proteins are clustered on the chloroplast genome of tobacco (Nicotiana tabacum): similarity to the S10 and spc operons of Escherichia coli.
- 1 August 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (16) , 6030-6034
- https://doi.org/10.1073/pnas.83.16.6030
Abstract
The nucleotide sequence of a tobacco (Nicotiana tabacum) chloroplast gene cluster that encodes eight proteins homologous to Escherichia coli ribosomal proteins L23, L2, S19, L22, S3, L16, L14, and S8 has been determined. RNA gel blot hybridization revealed that all eight coding regions are expressed in the chloroplasts. The arrangement of the eight genes resembles that found in the E. coli S10 and spc operons. Among the eight genes, the L2 and L16 genes contain 666- and 1020-base-pair introns, respectively. These intron boundary sequences are consistent with the conserved boundary sequences of the chloroplast group III introns [Shinozaki, K., Deno, H., Sugita, M., Kuramitsu, S. & Sugiura, M. (1986) Mol. Gen. Genet. 202, 1-5].This publication has 19 references indexed in Scilit:
- Structure of theEscherichia coliS10 ribosomal protein operonNucleic Acids Research, 1985
- Tobacco chloroplast tRNA Lys (UUU) gene contains a 2.5-kilobase-pair intron: An open reading frame and a conserved boundary sequence in the intronProceedings of the National Academy of Sciences, 1985
- STRUCTURE OF RIBOSOMAL RNAAnnual Review of Biochemistry, 1984
- Junctions of the large single copy region and the inverted repeats inSpinacia oleraceaandNicotiana debneyichloroplast DNA: sequence of the genes for tRNAHisand the ribosomal proteins S19 and L2Nucleic Acids Research, 1984
- The genes for the ribosomal proteins S12 and S7 are clustered with the gene for the EF-Tu protein on the chloroplast genome ofEuglena gracilisNucleic Acids Research, 1984
- Thespcrtbosomal protein operon ofEschenchia coli:sequence and cotranscriptlon of the rlbosomal protein genes and a protein export geneNucleic Acids Research, 1983
- A putative gene of tobacco chloroplast coding for ribosomal protein similar toE. coilribosomal protein S19Nucleic Acids Research, 1983
- Maize chloroplast DNA encodes a protein sequence homologous to the bacterial ribosome assembly protein S4Nucleic Acids Research, 1983
- Ribosomal components from Escherichia coli 50 S subunits involved in the reconstitution of peptidyltransferase activity.Journal of Biological Chemistry, 1981
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977