Microsequencing of dopamine beta‐monoozygenase

Abstract
Tryptic peptides and cyanogen bromide fragments of dopamine beta‐monooxygenase (DBH) were prepared and separated on C‐8 reverse phase columns by high pressure liquid chromatography. Absorbance profiles at both 220 nm and 280 nm were monitored so that peptides with aromatic residues could be isolated. These peptides were subjected to automated Edman degradation with a gas phase microsequencer.