Endotoxic Activity of L-Forms Derived from Salmonella paratyphi B.

Abstract
L-forms of Salmonella paratyphi B 777 were prepared in penicillin and maintained for several subcultures with and without the antibiotic. The toxicity of these L-forms was compared with the toxicity of parent bacterial cultures by intravenous injection into mice which had previously been infected with BCG and these were highly sensitive to endotoxin shock. L-forms were found to be 5.5 to 9 times less toxic than bacterial forms. The residual toxicity of L-forms remained constant during up to 6 subcultures in medium with or without penicillin.

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