LOCALIZATION OF FC AND FAB FRAGMENTS OF NONSPECIFIC POLYCLONAL IGG AT FOCAL SITES OF INFLAMMATION

  • 1 July 1990
    • journal article
    • research article
    • Vol. 31  (7) , 1199-1205
Abstract
Intact IgG, Fc, Fab, and 1/2Fc (reduced and alkylated Fc) were coupled to DTPA, labeled with indium-111 and administered to rats to compare the ability of fragments of IgG to localize at focal sites of inflammation. Two sets of experiments were performed: First, 1, 6, 24, and 48 hr after injection, biodistribution was determined in healthy animals; second, localization at sites of inflammation was determined by scintillation camera imaging of animals with deep-thigh infection due to Escherichia coli. The biodistribution studies demonstrated differences in kidney and liver localization: IgG < Fab < 1/2Fc (kidney), Fc < 1/2Fc < IgG < Fab (liver). The imaging studies revealed that target-to-background ratio (T/B) and percent residual activity (%RA) for IgG was significantly greater (p < 0.01) than 1/2Fc or Fab, and T/B for IgG was greater (p < 0.01) than Fc. These studies suggest that the Fc portion of IgG is the fragment with the best imaging properties.

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