CLINICAL APPLICATION OF THE TWO-LAYER (UNIVERSITY OF WISCONSIN SOLUTION/PERFLUOROCHEMICAL PLUS O 2) METHOD OF PANCREAS PRESERVATION BEFORE TRANSPLANTATION

1 September 2000

journal article
research article
Published by Wolters Kluwer Health in Transplantation

Vol. 70 (5) , 771-774
https://doi.org/10.1097/00007890-200009150-00010

Abstract

The two-layer method [University of Wisconson solution (UW)/perfluorochemical plus O 2] for pancreas preservation has been demonstrated to be superior to simple UW storage alone in the canine model.For the first time, we applied the two-layer method to clinical whole-pancreas transplantation. Pancreases were placed in the two-layer method in 10 cases and UW alone in 44 cases before transplant. The mean cold ischemic time was 16.5 hr in the two-layer group versus 18.1 hr in the UW group (P =NS). We compared the condition of graft at the time of reperfusion, and then 3 months posttransplant graft function and complications. At the time of reperfusion, no grafts in the two-layer group were edematous, compared with 10(23.3%) of 43 in the UW group (P =0.18). Seven (70%) of 10 grafts in the two-layer group obtained the best overall quality score, compared with 24(57.1%) of 42 in the UW group (P =0.72). Nine (90%) of 10 recipients in the two-layer group became insulin-independent during hospitalization, compared with 31(70.5%) of 44 in the UW group (P =0.26). Time to insulin independence was no different between the two groups. No pancreas grafts preserved by the two-layer method suffered acute rejection. The two-layer preservation method is feasible in human clinical transplantation. It was at least equivalent and may be superior to UW alone in both morphologic and functional assessment of the transplanted pancreas.

Keywords

UNIVERSITY OF WISCONSIN SOLUTION

This publication has 10 references indexed in Scilit:

PROLONGED PRESERVATION TIME INCREASES THE INCIDENCE OF SURGICAL COMPLICATIONS AFTER PANCREAS TRANSPLANT
Transplantation, 1999
THE EFFECT OF OUABAIN (INHIBITOR OF AN ATP-DEPENDENT NA+/K+ PUMP) ON THE PANCREAS GRAFT DURING PRESERVATION BY THE TWO-LAYER METHOD1
Transplantation, 1996
DIRECT EVIDENCE OF PANCREATIC TISSUE OXYGENATION DURING PRESERVATION BY THE TWO-LAYER METHOD
Transplantation, 1996
RESUSCITATION OF ISCHEMICALLY DAMAGED PANCREAS DURING SHORT-TERM PRESERVATION AT 20??C BY THE TWO-LAYER (UNIVERSITY OF WISCONSIN SOLUTION/PERFLUOROCHEMICAL) METHOD
Transplantation, 1996
Successful Extended Preservation Of Ischemically Damaged Pancreas By The Two-LaYER (UNIVERSITY OF WISCONSIN SOLUTION/PERFLUOROCHEMICAL) COLD STORAGE METHOD
Transplantation, 1993
RESTORATION OP PANCREAS GRAFT FUNCTION PRESERVED BY A TWO-LAYER (UNIVERSITY OF WISCONSIN SOLUTION/PERFLUOROCHEMICAL) COLD STORAGE METHOD AFTER SIGNIFICANT WARM ISCHEMIA
Transplantation, 1993
OXYGENATION OF THE HUMAN PANCREAS DURING PRESERVATION BY A TWO-LAYER (UNIVERSITY OF WISCONSIN SOLUTION/PERFLUOROCHEMICAL) COLD-STORAGE METHOD
Transplantation, 1992
Local T cell responses induce widespread MHC expression. Evidence that IFN-gamma induces its own expression in remote sites.
The Journal of Immunology, 1992
PRESERVATION OF CANINE PANCREAS FOR 96 HOURS BY A MODIFIED TWO-LAYER (UW SOLUTION/ PERFLUOROCHEMICAL) COLD STORAGE METHOD
Transplantation, 1991
SEVENTY-TWO-HOUR PRESERVATION OF THE CANINE PANCREAS BY THE TWO-LAYER (EURO-COLLINSʼ SOLUTION/ PERFLUOROCHEMICAL) COLD STORAGE METHOD
Transplantation, 1989