SOME PROPERTIES OF MARROW DERIVED ADHERENT CELLS IN TISSUE-CULTURE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 56  (6) , 1006-1012
Abstract
Monolayer cultures of marrow derived adherent cells (MDAC), apparently consisting of fibroblasts, macrophages, epithelioid cells and fat cells, can support long-term stem cell proliferation in vitro. The hematopoietic support capability of murine MDAC monolayers was confirmed and the cultured cells further characterized with respect to the following properties: esterase I activity, complement component 3 (C3) receptors, IgG (Fc) receptors, colony stimulating activity (CSA) production, and collagen synthesis. The cultures were also examined immunohistochemically to localize fibronectin, laminin and collagen synthesis and to identify the collagen subtypes synthesized. MDAC morphology was as described in previous studies, although fat cells were few in number. MDAC included some cells with esterase I activity and C3 receptors. Fc receptors were not detected and the cultures did not produce CSA, indicating that mononuclear phagocytes were not present. MDAC synthesized fibronectin and collagen types I and III. Staining for epithelial basement membrane proteins (collagen types IV and V and laminin) was negative. The vast majority of these cultured MDAC were apparently fibroblasts.