MULTIPLE FORMS OF HUMAN GAMMA-GLUTAMYLTRANSFERASE - PREPARATION AND CHARACTERIZATION OF DIFFERENT MOLECULAR-WEIGHT FRACTIONS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 27  (1) , 1-8
Abstract
Eight fractions of human .gamma.-glutamyltransferase were prepared from liver tissue, serum and bile by gel filtration. Bile, pooled serum from patients with high .gamma.-glutamyltransferase activities and serum in which liver tissue had been incubated each contained an enzyme fraction with MW > 106. A fraction of .apprx. 80,000 MW was obtained from bile, by incubation of liver tissue or sodium chloride solution but not from the serum pool. The main enzyme fraction in native serum had a MW of .apprx. 300,000, and the MW of .gamma.-glutamyltransferase partially purified from liver was initially 160,000. The fractions had similar Km and Ki values, and differences in heat stability and binding to concanavalin A were not marked.

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