THE PROPAGATION OF THE VIRUS OF EPIZOOTIC HEMORRHAGIC DISEASE OF DEER IN NEWBORN MICE AND HELA CELLS

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Abstract
A complement-fixing antigen prepared from the brains of infected mice reacts positively with the sera of deer recovered from infection with either the New Jersey or South Dakota strain of virus, but not with the serum of normal deer. The mouse-passaged virus induced an inapparent infection in an experimental deer. The virus can be grown serially in He La cell culture and induces a characteristic cytopathic effect It is neutralizable in such cultures to high titer by the sera of deer recovered from EHD (New Jersey strain) and to lower titer by the serum of a deer recovered from EHD (South Dakota strain) but not by normal deer serum. The HeLa cell-passaged virus induced typical lethal EHD in an experimental deer and virus could be recovered in HeLa cell culture. The EHD virus was sensitive to sodium desoxycholate which suggests that it may belong in the arbor virus group.