MORPHOLOGY OF BOVINE VIRAL DIARRHEA VIRUS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 45  (5) , 845-850
Abstract
The morphology of bovine viral diarrhea virus (BVDV) was studied by EM. The NADL strain of BVDV was plaque purified 3-fold, concentrated by polyethylene glycol precipitation and purified by centrifugation to equilibrium in continuous potassium tartrate density-gradients. The virus was examined by negative-stain EM in the presence or absence of specific antiserum. The density of BVDV was 1.101-1.174 g/cm3, with the peak at maximum infectivity at 1.122 g/cm3. Oval to pleomorphic viral particles, 120 (.+-. 30) nm in diameter, were enriched in the peak of maximum infectivity. The detailed structure of virions was revealed: a 5- to 7-.mu.m thick unit membrane-like envelope layer with numerous projecting knobs, 4-5 nm in diameter, surrounding an interior core-like structure. Viral particles measuring 120 (.+-. 30) nm were found in large aggregates in the presence of specific antiserum.

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