3H nuclear magnetic resonance study of anaerobic glycolysis in packed erythrocytes.
- 1 January 1990
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 87 (2) , 583-587
- https://doi.org/10.1073/pnas.87.2.583
Abstract
The utility and power of 3H NMR spectroscopy as a technique for monitoring biological systems in vivo is illustrated with glucose metabolism in erythrocytes. Use of C-1-tritiated glucose allowed us to monitor the disappearance of the alpha and beta tritons, with the production of lactate and 1H3HO (HTO), as well as some intermediates. Spin lattice relaxation times (T1) were measured to avoid T1 distortion of the spectral intensities. Detection of the formation of 1 mM tritiated water in the presence of 110 M H2O protons and deuterons allows the eventual fate of the label in the pentose shunt to be observed in vivo.This publication has 11 references indexed in Scilit:
- Biosynthesis of porphyrins and corrins. 2. Isolation, purification, and NMR investigations of the porphobilinogen deaminase covalent complexBiochemistry, 1986
- Regulation of the human‐erythrocyte hexose‐monophosphate shunt under conditions of oxidative stressEuropean Journal of Biochemistry, 1985
- A multinuclear NMR study of 2,3-bisphosphoglycerate metabolism in the human erythrocyteBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1984
- Interaction of purified yeast cytochrome P-450 and labelled benzo[a]pyrene studied by tritium nuclear-magnetic-resonance spectroscopy [proceedings].1980
- 13C nuclear magnetic resonance studies of anaerobic glycolysis in suspensions of yeast cells.Proceedings of the National Academy of Sciences, 1979
- Cellular Applications of 31 P and 13 C Nuclear Magnetic ResonanceScience, 1979
- Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transportBiochemical Journal, 1979
- High-resolution 13C nuclear magnetic resonance studies of glucose metabolism in Escherichia coli.Proceedings of the National Academy of Sciences, 1978
- Tritium nuclear magnetic resonance spectroscopy. Part 7. New information from the tritium distribution in biosynthetically labelled penicillic acidJournal of the Chemical Society, Perkin Transactions 1, 1977
- Studies on Erythrocyte GlycolysisThe Journal of Biochemistry, 1966