CARCINOGENICITIES OF 3-METHOXY-4-AMINOAZOBENZENE, N-HYDROXY-3-METHOXY-4-AMINOAZOBENZENE AND RELATED AZO DYES IN THE MOUSE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 73  (1) , 136-140
Abstract
The carcinogenicities of 2-methoxy-4-aminoazobenzene and 3-methoxy-4-aminoazobenzene (2-MeO-AAB and 3-MeO-AAB) and their N-hydroxy derivatives (N-OH-2-MeO-AAB and N-OH-3-MeO-AAB) were tested in (C3H .times. C57BL/6) F1 mice. Topical s.c. injections, twice weekly for 8 wk, of 4 or 8 .mu.mol of N-OH-3-MeO-AAB dissolved in 0.1 ml of olive oil containing 10% dimethyl sulfoxide induced fibrosarcomas at the site of application. Similar treatment with 3-MeO-AAB or 2-MeO-AAB as well as N-OH-2-MeO-AAB failed to induce sarcomas. Oral administration of 0.09% 3-MeO-AAB in the diet for 13 mo. induced hepatic tumors in female mice but not in males. 2-MeO-AAB did not induce tumors in male or female mice. The relationship between carcinogenicity, mutagenicity, the ability to induce unscheduled DNA synthesis, and the metabolism of these azo dyes are discussed.