Decrease in CuZn‐superoxide dismutase mRNA level during differentiation of human monocytic and promyelotic leukemia cells
- 5 June 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (2) , 253-256
- https://doi.org/10.1016/0014-5793(89)80634-9
Abstract
Change in the level of CuZn‐superoxide dismutase (SOD) mRNA was examined using a molecular probe during differentiation of human monocytic leukemia U937 cells or promyelotic leukemia HL‐60 cells induced by either 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA) or dimethylsulfoxide (DMSO). CuZn‐SOD mRNA levels were found to decrease during the course of differentiation, and this response is specific for differentiation, since the treatment of human B cell leukemia cells or normal diploid fibroblasts with TPA failed to have any effect on the level of CuZn‐SOD mRNA. The activity of CuZn‐SOD in U937 cells also decreased during differentiation, but following that of the CuZn‐SOD mRNA level. The expression of the CuZn‐SOD gene is thus concluded to diminish during the differentiation of HL‐60 and U937 cells.Keywords
This publication has 27 references indexed in Scilit:
- Superoxide as a signal for increase in intracellular pHJournal of Cellular Physiology, 1988
- Induction of c-sis gene expression and synthesis of platelet-derived growth factor in human myeloid leukemia cells during monocytic differentiation.Proceedings of the National Academy of Sciences, 1986
- Human Leukemic Models of Myelomonocytic Development: A Review of the HL-60 and U937 Cell LinesJournal of Leukocyte Biology, 1985
- Prooxidant States and Tumor PromotionScience, 1985
- Modulation of C-myc expression in the HL-60 cell lineBiochemical and Biophysical Research Communications, 1984
- Regulation of myc gene expression in HL-60 leukaemia cells by a vitamin D metaboliteNature, 1983
- Differential expression of the amv gene in human hematopoietic cells.Proceedings of the National Academy of Sciences, 1982
- Activity and activation of the granulocyte superoxide-generating systemBlood, 1980
- Continuous growth and differentiation of human myeloid leukaemic cells in suspension cultureNature, 1977
- Establishment and characterization of a human histiocytic lymphoma cell line (U‐937)International Journal of Cancer, 1976