Comparison of Latex Agglutination, Indirect Hemagglutination, and ELISA Techniques for the Detection ofToxoplasma gondii‐specificAntibodies in the Serum of Cats

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Abstract
The primary purpose of this study was to determine whether commercially available latex agglutination and indirect hemagglutination kits for the detection ofToxoplasma gondii‐specificantibodies were capable of detectingT. gondii‐specificimmunoglobulin M (IgM) in the serum of cats. Serum samples from 35 cats containing eitherT. gondii‐specificIgM,T. gondii‐specificimmunoglobulin G (IgG), or both were collected. Each serum sample was assayed using a latex agglutination kit, an indirect hemagglutination kit, an enzyme‐linked immunosorbent assay (ELISA) for the detection ofT. gondii‐specificIgG, and an ELISA for the detection ofT. gondii‐specificIgM. When serum samples containing onlyT. gondii‐specificIgM as determined by ELISA were assayed, the latex agglutination kit and the indirect hemagglutination kit detected antibodies in 33.3% and 13.3%, respectively. WhenT. gondii‐specificIgG was present in a serum sample, the results from the latex agglutination kit, the indirect hemagglutination kit, and the IgG‐ELISA were similar; however, there was a wide variation in titer magnitude results between the three assays. It was concluded that the latex agglutination kit and the indirect hemagglutination kit did not adequately detectT. gondii‐specificIgM in feline serum.