Hydrolysis of ε-Amincaproyl Compounds by Trypsin*

Abstract
It has been known that trypsin, an important proteolytic enzyme, can hydrolysis only lysyl and arginyl peptide, amide, and ester linkages (1, 2), Recently it has been shown that benzoyl-l-histidine ester (3) and n-fatty acid esters (4) are also susceptible to the action of the enzyme. Shapiro and Doherty (5) have briefly reported that ε-aminocaproic acid esters can also be hydrolysed by trypsin. The author has independently found in the course of a study on tryptic hydrolysis of poly-ε-aminocaproyl-α-alanines (6) that the ε-aminocaproyl peptides, amides and esters are hydrolysed by the action of trypsin. The present paper deals with detailed studies concerning the hydrolysis of the ε-aminocaproyl compounds by the action of trypsin.