Mass transfer effects in microencapsulated hybridoma cells producing monoclonal antibodies
- 1 January 1989
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 20-21 (1) , 603-619
- https://doi.org/10.1007/bf02936512
Abstract
Rat-mouse and mouse-mouse hybridoma cell lines were used for formation of monoclonal antibodies (MAbs) in microcapsules of different sizes. Microcapsules were made of poly L-lysine-alginate hydrogel membranes. The effects of extracapsule liquid film, intra-capsule and transmembrane transfer limitations of nutrients/products on system’s performance were investigated. An agitation speed of 45 rpm (4 cm/s tip speed) was found to be optimal in spinner flasks to overcome liquid film resistances around capsules. Capsule sizes need to be reduced to smaller than 350 ε in order to eliminate intracapsule transfer limitations with a typical initial viable cell concentration of 0.5x105 viable cells/mL capsule. Double coating of capsules to improve strength of capsules resulted in higher transmembrane transfer resistances.Keywords
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