INVIVO CELL-CYCLE PHASE-PREFERENTIAL KILLING OF MURINE FIBRO-SARCOMA CELLS BY CISPLATIN

  • 1 January 1981
    • journal article
    • research article
    • Vol. 65  (9-10) , 845-851
Abstract
Fibrosarcoma [methylcholanthrene-induced FSa] cells were separated by centrifugal elutriation. Selected populations enriched in G1-, S-, or G2 + M-phase cells were then injected (104 cells) into the tail veins of C3Hf/Kam mice. At 20 min later, 4 mg/kg of cisplatin was injected i.v. into 1/2 the animals in each experimental group. Fourteen days later the number of lung colonies was scored; the surviving fractions were plotted against the elutriator fraction numbers. All phases of the cell cycle were sensitive to cisplatin but the G1 phase was the most sensitive by a factor of 10. Biphasic dose-response curves were observed for unseparated control, S-phase and G2 + M-phase tumor cell populations, indicating a resistant cell population. G1 cells had log killing over 4 decades without the resistant tail. Using this in vivo system, cisplatin apparently kills cells throughout the cell cycle but exhibits preferential killing of G1 tumor cells.

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