Granulocyte Isolation from Whole Blood of Goat, Sheep, Cattle, Horse, Dog, Pig, and Man
- 12 January 1989
- journal article
- research article
- Published by Wiley in Journal of Veterinary Medicine, Series B
- Vol. 36 (1-10) , 191-198
- https://doi.org/10.1111/j.1439-0450.1989.tb00591.x
Abstract
A simple two step procedure for the isolation of caprine, ovine, bovine, equine, canine, porcine and human peripheral blood granulocytes is described. After enrichement of granulocytes by centrifugation, contaminating erythrocytes are lysed hypotonically. Recovery, purity and viability of the granulocyte suspensions are determined. FACScan analysis of the cell suspensions measuring cellular size by forward and sideward light scatter is compared with the corresponding analysis of whole blood leukocytes. Constituencies of the isolated cell suspensions and loss of granulocyte subpopulations through isolation procedure is discussed with regard to granulocyte function assays.Keywords
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