Autonomous Replication and Expression of RNA 1 from Black Beetle Virus

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Abstract
Black beetle virions contain 2 RNA. The smaller one, RNA 2, has previously been shown to be a messenger for viral coat protein. It is shown here, by infecting sensitized Drosophila cells with the individually purified RNA, that the larger one, RNA 1, carries the viral gene(s) required for RNA polymerase functions. RNA 2 was dispensible for synthesis of viral RNA 1 and subgenomic RNA 3 but was essential for synthesis of RNA 2 and virions. Cells infected with RNA 1 alone produced RNA 3 in proportions 10- to 20-fold greater than cells infected with virions. This overproduction of RNA 3 decreased with increasing proportions of RNA 2 in the infecting RNA 1. Evidently, RNA 1 is the previously unidentified progenitor of subgenomic RNA 3, RNA 2 regulates the amount of RNA 3 produced in the infected cell.