In vitro enhancement of the mutagenicity of 4‐nitro‐o‐phenylenediamine by plant S‐9
- 1 January 1985
- journal article
- research article
- Published by Wiley in Environmental Mutagenesis
- Vol. 7 (1) , 73-85
- https://doi.org/10.1002/em.2860070104
Abstract
The direct-acting mutagen 4-nitro-o-phenylenediamine (NOP) was activated in vitro by pea or tobacco S-9 into a more potent mutagen in Salmonella typhimurium strain TA98. NOP mutagenicity was not altered by Aroclor 1254-induced rat liver S-9. The plant S-9 activation of NOP was heat-sensitive but was not NADPH-dependent, did not involve superoxide radicals, and was not inhibited by CO. A direct relationship between plant peroxidase and NOP activation was established. Several purified peroxidases including horseradish peroxidase, chloroperoxidase, and lactoperoxidase also activated NOP. The perodoxidative process was not H2O2-dependent but was partially inhibited by a peroxidase inhibitor.Keywords
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