Antiproliferative Effects of the Readily Extractable Fractions Prepared from Various Citrus Juices on Several Cancer Cell Lines

Abstract
To eliminate the masking effect by flavonoid glycosides, which comprise ∼70% of conventionally prepared sample, the readily extractable fraction from Citrus juice, which was prepared by adsorbing on HP-20 resin and eluting with ethanol and acetone from the resin, was subjected to antiproliferative tests against several cancer cell lines. Screening of 34 Citrus juices indicated that King (Citrus nobilis) strongly inhibited proliferation of all cancer cell lines examined. Sweet lime and Kabuchi inhibited three of the four cancer cell lines. In contrast, these samples were substantially less cytotoxic toward normal human cell lines. Keywords: Citrus; readily extractable fraction; A549; B16 melanoma 4A5; CCRF-HSB-2; TGBC11TKB; human foreskin keratinocytes; human umbilical vein endothelial cells

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