Reciprocal Protective Immunity againstBordetella pertussisandBordetella parapertussisin a Murine Model of Respiratory Infection

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Abstract
The protective immunity induced by infection withBordetella pertussisand withBordetella parapertussiswas examined in a murine model of respiratory infection. Convalescent mice that had been infected by aerosol withB. pertussisor withB. parapertussisexhibited a protective immune response againstB. pertussisand also againstB. parapertussis. Anti-filamentous hemagglutinin (anti-FHA) serum immunoglobulin G (IgG) and anti-FHA lung IgA antibodies were detected in both mice infected withB. pertussisand those infected withB. parapertussis. Antibodies against pertussis toxin (anti-PT) and against killedB. pertussiscells were detected in mice infected withB. pertussis. Pertactin-specific antibodies and antibodies against killedB. parapertussiscells were detected in mice infected withB. parapertussis. Spleen cells from mice infected withB. pertussissecreted interferon-γ (IFN-γ) in response to stimulation by FHA or PT. Spleen cells from mice infected withB. parapertussisalso secreted IFN-γ in response to FHA. Interleukin-4 was not produced in response to any of the antigens tested. The profiles of cytokine secretion in vitro revealed induction of a Th1-biased immune response during convalescence from infection byB. pertussisand byB. parapertussis. It is possible that Th1 and Th2 responses against FHA might be related to the reciprocal protection achieved in our murine model.

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