3-Hexulose phosphate synthase from a new facultative methylotroph, Mycobacterium gastri MB19.
Open Access
- 1 January 1988
- journal article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 52 (10) , 2659-2661
- https://doi.org/10.1271/bbb1961.52.2659
Abstract
A newly found methanol-using bacterium, Mycobacterium gastri MB 19, is a facultative methylotroph which assimilates methanol via the ribulose monophosphate pathway. 3-Hexulose phosphate synthase was purified from the organism and characterized. This enzyme was found to use glycolaldehyde (Km =4.3 mM) and methylglyoxal (Km=5.7 mM) as well as formaldehyde (Km=1.4 mM) in the presence of D-ribulose 5-phosphate as an acceptor. The product of the condensation of glycolaldehyde with D-ribulose 5-phosphate was isolated by ionexchange chromatography. The dephosphorylated product was tentatively identified as a heptulose with the molecular formula C7H14O7 from its spectrophotometric properties and GC-MS results.Keywords
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