INCREASE OF THE (NA++K+)-ACTIVATED ATPASE ACTIVITY OF BASOLATERAL PLASMA-MEMBRANES FROM INTESTINAL-MUCOSA OF DIABETIC RATS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 41  (10) , 891-898
Abstract
A significant increase in (Na+ + K+)-activated ATPase was found in mucosal homogenates of rat small intestine under conditions of alloxan and streptozotocin diabetes. From studies with isolated plasma membranes it has been shown that the activity changes were caused by the part of the (Na+ + K+)-activated ATPase which is only localized in the basolateral plasma membrane, whereas the enzyme activity in the brush border region remains unchanged. In connection with the enhanced capacity of ion, nonelectrolyte and water absorption in experimental diabetes, the concept of intestinal transport mechanism which suggests that the basolateral part of the (Na+ + K+)-activated ATPase is responsible for metabolic energy supply is supported. The luminal part of the enzyme may be involved in the regulation of passive Na+ influx.