Fluorescence properties of indocyanine green as related to angiography

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Abstract
Indocyanine green (ICG) absorption and fluorescence angiography are relatively new techniques to study the choroidal circulation. Accurate information regarding the absorption and fluorescence properties of ICG in human whole blood is essential to obtain good angiograms, especially of the smaller vessels. Since ICG has been extensively used in determining cardiac output and hepatic function, the absorption properties of ICG have been studied, mainly with regard to the effects of dye aggregation and binding to plasma protein. A similar study of the fluorescence properties of ICG (spectrum, concentration dependence and quantum yield) was done. The fluorescence spectrum was measured as a function of dye concentration in human whole blood and a few other common solvents in order to determine what fluorescence properties are unique to ICG in blood.