Rapid biochemical screening for Salmonella, Shigella, Yersinia, and Aeromonas isolates from stool specimens
- 1 June 1994
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 32 (6) , 1583-1585
- https://doi.org/10.1128/jcm.32.6.1583-1585.1994
Abstract
Four screens for the rapid (4 to 6 h) biochemical detection of pathogens from enteric isolation media are described. The Salmonella screen consisted of Kligler iron agar (KIA), motility-indole-urea-tryptophan-deamination semisolid medium (MIU-TDA), and the o-nitrophenyl-beta-D-galactopyranoside (ONPG) test; the Shigella screen consisted of KIA, MIU-TDA, the ONPG test, and the lysine decarboxylation-indole test; the Yersinia screen consisted of a rhamnose broth; the Aeromonas screen consisted of a xylose agar plate. When tested on 2,102 fresh isolates and 71 stock strains, the screens correctly detected 212 enteric pathogens (sensitivity, 100%), with a specificity of 98.1%.Keywords
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