An experimental study of enhanced cell killing by hyperthermia and bleomycin

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Abstract
Summary In vitro cell killing effects from heating and/or bleomycin have been investigated using the KK-47 cell line derived from a human bladder carcinoma and the colonyforming assay system. Percent survivals of the asynchronous cells heated to 40°, 41°, 42° and 43°C for 30 min were 96.6%, 93.3%, 91.4% and 71.2%, respectively, and those at 43° C for 2 h were 80.6%, 75.5%, 46.0% and 1.4%, respectively. The survival percentages of the cells treated with bleomycin at the concentrations of 1, 3, 6 and 10 μg per ml at 37°C for 30 min were 66.0%, 40.4%, 21.9% and 11.9%, respectively. When hyperthermia at 43°C was combined with bleomycin, a greatly enhanced cell killing effect was obtained by preheating and simultaneous heating as compared with the effect of post-heating.