Pyridine Nucleotides in the Thyroid Gland. VII. Effect of Thyroid Stimulating Hormone on ATP Concentration in Dog Thyroid Slices

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Abstract
The ATP concentration of dog thyroid slices averages 319 [plus or minus] 76 m[mu] moles/g wet weight of tissue with a range from 167-669 m[mu]moles/g. Incubation of thyroid slices with TSH decreased the ATP levels during a 45-min. incubation. This reduction was significantly greater when glucose was omitted from the incubation medium. A 45-min. incubation period by itself did not change the ATP concentrations of thyroid slices. Iodoacetate, dicumarol, dinitrophenol, NaF and para-hydroxymercuri-benzoate all decreased the basal and TSH-stimulated glucose-1-C14 oxidation and caused marked reduction of ATP levels in thyroid slices. There was no apparent correlation between C14O2 production and ATP levels. The present results indicate that the TSH-mediated increase in TPN is not due to an effect of the hormone on increasing ATP concentrations.