Affinity Labeling of Adenosine A1 Binding Sites
- 1 September 1987
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 49 (3) , 681-684
- https://doi.org/10.1111/j.1471-4159.1987.tb00947.x
Abstract
The adenosine A1 receptors of sheep brain membranes have been identified by the specific binding of radiolabeled cyclohexyl [3H]adenosine ([3H]CHA). Pretreatment of membranes with periodate-oxidized CHA causes a dose-and time-dependent decrease in the number of binding sites. No decrease occurs when membranes are pretreated with CHA. Binding of [3H]CHA to the remaining sites occurs with the same characteristics as binding to the untreated receptor population.Keywords
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