CULTURE OF HUMAN BONE MARROW

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Abstract
In this report we describe briefly a method for the culture of human marrow in quantities permitting hematologic and metabolic studies. We also outline some of the problems under investigation by this method. The apparatus supplies a lung, kidney and circulation for the marrow, as it provides for the control of oxygen and carbon dioxide tension, ofpH, and of the composition of the medium; for elimination of waste products, for supply of nutrients, for removal of part or all of the culture for study, and for maintenance of sterility. The most important feature of the apparatus is a semipermeable membrane, separating the culture from the main volume of medium. This membrane allows nutrient materials from the surrounding medium to diffuse into the culture as needed and allows waste products to diffuse out as they accumulate. Because of this equilibrium, analysis of the outflowing medium gives a good indication of

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