Activity of Hydra Cells in vitro and in Regenerating Cell Reaggregates
Open Access
- 1 May 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in American Zoologist
- Vol. 14 (2) , 543-550
- https://doi.org/10.1093/icb/14.2.543
Abstract
Hydra were disaggregated into single cells and maintained in a complex medium for a few days. The cells are capable of macromolecular synthesis, of processing RNA, and of carrying out the formation of a nematocyst capsule. Single cells can be centrifuged into aggregates which will develop into normal hydra. Changes in morphology and cell composition are described. Interstitial cells after a 1-day delay resume their normal mitotic and differentiation activity in the aggregates. The method provides new means for studying problems of cell differentiation in hydra.Keywords
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