IN VITROTHYROXINE EFFECT ON RAT KIDNEY CORTEX AS INFLUENCED BY ADDITION OF AMINO ACIDS1

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Abstract
The thyroxine maintenance of the metabolism of rat kidney slices incubated at 5[degree] C for 24, 48 and 72 hours has been studied in terms of specific participation of some 50 amino acids and related compounds. Based on a comparison of initial QO2 and maintenance with similar values obtained with simple Ringer-glucose solution without added amino acid, DL-alanine, N-acetyl-DL-alanine, and L-proline were the most active. Considered as intermediate were [beta]-alanine, threonine, [beta]- and [gamma] -amino-n-butyric acids, taurine, [alpha]- and [beta]-amino-isobutyric acids. Tryptophan inhibited the thyroxine effect, alone or in the presence of alanine or proline. Further analysis of the DL-alanine action indicated the D-form to be responsible for all of the activity. Indirect evidence points to deamination plus decarboxylation being important in the alanine effect, but transamination and even peptide bond synthesis have not been ruled out.

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