Isolation and functional characterization of hemoglobin Casper. β106(G8)Leu → Pro
- 4 November 1975
- journal article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 14 (22) , 5017-5020
- https://doi.org/10.1021/bi00693a036
Abstract
Hemoglobin Casper (beta106Leu replaced by Pro) can be separated from hemoglobin (Hb) A by isoelectric focusing on polyacrylamide gel. This abnormal hemoglobin was estimated to be 30% of teh total by both isoelectric focusing and heat lability kinetics. Its oxygen equilibrium curves indicate a high oxygen affinity, low degree of subunit interaction, and a decreased Bohr effect. Mixtures of Hb Casper and Hb A appear to bind oxygen as if no hybrid molecules exist.Keywords
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