ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTING ANTIBODIES TO BRUCELLA-ABORTUS IN BOVINE-MILK AND SERUM
- 1 January 1980
- journal article
- research article
- Vol. 41 (12) , 2082-2084
Abstract
An enzyme-linked immunosorbent assay (ELISA) method was evaluated for the detection of antibodies to B. abortus in cow''s milk. Milk samples from seropositive or -negative cows were used to determine the distribution of absorbance values to classify milk as ELISA positive or ELISA negative. B. abortus was isolated from milk samples from 10 (45%) of the 22 cows whose milk and serum were ELISA positive. The ELISA was evaluated and determined to be an appropriate method for detecting antibodies to B. abortus in bovine milk.This publication has 6 references indexed in Scilit:
- Interpretation of spectrophotometric absorbance values to define results of enzyme-linked immunosorbent assaysJournal of Clinical Microbiology, 1980
- Comparison of serological methods for the detection of B. abortus antibodies in sera from vaccinated and non-vaccinated cattleEpidemiology and Infection, 1979
- Enzyme immunoassay for detecting Brucella antibodies in cow's milkJournal of Clinical Microbiology, 1979
- Brucella abortus IN COYOTES. I. A SEROLOGIC AND BACTERIOLOGIC SURVEY IN EASTERN TEXASJournal of Wildlife Diseases, 1979
- The Nature of the Local Immune System of the Bovine Mammary GlandThe Journal of Immunology, 1977
- BACTERIOLOGIC EVALUATION OF SEROLOGIC TEST PROCEDURES FOR DIAGNOSIS OF BRUCELLOSIS IN PROBLEM CATTLE HERDS1966