Effect of IFN-γ treatment andin vivo passage of murine tumor cell lines on their sensitivity to lymphokine-activated killef (LAK) cell lysisin vitro; association with H-2 expression on the target cells

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Abstract
Interferon-gamma (IFN-γ) treatment or in vivo passage of the murine YAC-I lymphoma resulted in reduced sensitivity to in vitro lysis by syngeneic murine spleen cells cultured in rlL-2 (LAK-cells). IFN-γ treatment also rendered the murine BI6 melanoma less sensitive to lysis by syngeneic LAK cells, whereas in vivo passage did not alter LAK sensitivity. The reduction in sensitivity to lysis correlated with enhanced expression of cell surface H-2 on the target cells. The possible role of H-2 was studied with a β2-microglobulin-deficient, and thus H-2-deficient, variant of the YAC-I lymphoma. This variant line remained H-2 negative even after IFN-γ treatment or in vivo passage, and was highly sensitive to LAK-cell-mediated lysis, even after IFN-γ treatment or in vivo passage. The present results are discussed in relation to IFN-γ and in vivo induced modulation of MHC class-l molecules on target cells and the possible consequences for interaction with activated as well as “natural” effector cells.

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